European Impatiens species differences at RAPD and ISSR loci

Edita Ramonienė, Lina Jocienė, Algimantas Paulauskas, Eugenija Kupčinskienė
2016 Biologija  
Information concerning comparison of three widely spread European species of Impatiens along wider geographical areas is still missing. The present study is aimed at comparing genetic variability at RAPD and ISSR loci of Impatiens noli-tangere, I. parviflora, and I. glandulifera, covering a marked geographic area. Twenty four populations of these Impatiens (eight populations of each species) from two countries (Lithuania and Czech Republic) were examined. Eight randomly amplified polymorphic
more » ... fied polymorphic DNA (RAPD) and 5 inter simple sequence repeat (ISSR) markers were chosen considering the lack of data on the general molecular characteristics of Impatiens. The highest genetic differentiation at RAPD loci (GST = 0.81) was characteristic of I. parviflora, and the highest genetic differentiation at ISSR loci (GST = 0.73) was documented for I. glandulifera. According to Nei's genetic distances between two species populations, significant correlations were determined for I. noli-tangere and I. parviflora (r = 0.79; p
doi:10.6001/biologija.v62i1.3287 fatcat:bthq32cf7veejjeow7klrx5bgu