Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome

Andrew M. Lewis, Wallace P. Rowe
1973 Journal of Virology  
Two of the five nondef'ective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrids induce SV40 transplantation resistance in immunized hamsters. These two hybrids, Ad2+ND2 and Ad2+ND4, contain 32 and 43% of the SV40 genome, respectively. The pattern of' induction of' SV40 transplantation antigen (TSTA) by the various hybrids diff'erentiates TSTA from both SV40 U and T antigens. Since the SV40 RNA induced by both these hybrids is early SV40 RNA, these f'indings conf'irm that TSTA is an early SV40
more » ... unction. By combining available data on SV40 antigen induction by these hybrids with electron microscopy heteroduplex mapping studies, the DNA segment responsible for the induction of SV40 TSTA can be inf'erred to lie in the region between 0.17 and 0.43 SV40 units from the site on the SV40 chromosome cleaved by E. coli Rl restriction endonuclease. on May 9, 2020 by guest Downloaded from NONDEFECTIVE ADENOVIRUS-SV40 HYBRIDS. VIII.
doi:10.1128/jvi.12.4.836-840.1973 fatcat:dvxhi72wfnawdhpmc3uzdgspvi