We have developed a simple assay technology that enables measurement of the substrates used by eukaryotic cells and by their mitochondria to produce energy. The newest mitochondrial assays measure more than 50 aspects of mitochondrial function, profiling the rates of metabolism of mitochondrial substrates and the sensitivity of metabolism of these substrates to mitochondrial inhibitors. Using this technology one can scan cells to determine which pathways are operative and how the pathway

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... ies change due to genetic alterations, epigenetic gene regulation, and exposure to drugs or hormones. This presentation will review the technology and show examples of how it can inform basic and advanced cellular studies. SESSION 4 55ASM-0284 S1 PC2 | Measuring the impact of microbiome metabolites on mitochondrial function using the Agilent Seahorse XF analyser A. Caro-Maldonado Seahorse Product Specialist in Agilent Technologies | 13 ABSTRACT 55ASM-0276 PC S4 |