Precision genetic modifications: a new era in molecular biology and crop improvement

Franziska Fichtner, Reynel Urrea Castellanos, Bekir Ülker
2014 Planta  
Precision genetic modification techniques Using precision genetic modification (Pgm) techniques, researchers can target specific sequences in complex genomes and precisely introduce modifications including single nucleotide changes. Implementation of these techniques required several breakthroughs, including the ability to engineer recognition of a specific DnA sequence and generate a specific double-stranded DnA break (DsB). homology-directed repair (hDr) of this site-specific DsB can then
more » ... uce various alterations in the sequence Abstract recently, the use of programmable DnAbinding proteins such as ZFP/ZFns, TAlE/TAlEns and crIsPr/cas has produced unprecedented advances in gene targeting and genome editing in prokaryotes and eukaryotes. These advances allow researchers to specifically alter genes, reprogram epigenetic marks, generate site-specific deletions and potentially cure diseases. Unlike previous methods, these precision genetic modification techniques (Pgms) are specific, efficient, easy to use and economical. here we discuss the capabilities and pitfalls of Pgms and highlight the recent, exciting applications of Pgms in molecular biology and crop genetic engineering. Further improvement of the efficiency and precision of Pgm techniques will enable researchers to precisely alter gene expression and biological/chemical pathways, probe gene function, modify epigenetic marks and improve crops by increasing yield, quality and tolerance to limiting biotic and abiotic stress conditions.
doi:10.1007/s00425-014-2029-y pmid:24510124 fatcat:ofwexpfyfzdsta7qhpxxamn6hq