Effect of ethyl acetate extract from husk fiber water of Cocos nucifera in Leishmania braziliensis infected hamsters

José C. C. Freitas, Diana C. S. Nunes-Pinheiro, Adriana W.P. Pessoa, Luis C. R. Silva, Virgínia C. C. Girão, Belarmino E. Lopes-Neto, Michelle S. Agostinho, Cyntia R. A. Abreu
2011 Revista Brasileira de Farmacognosia  
The objective of this study was to evaluate the treatment with ethyl acetate extract (EAE) from husk fiber water of Cocos nucifera L., Arecaceae, in L. braziliensis (Lb) infected hamsters. Twelve male hamsters were randomly allocated in three groups (n=4): G1 received only EAE; G2 was infected with Lb only and G3 received EAE after Lb infection. The infection was carried 28 days prior to the treatment with EAE, which was administrated (0.2 mL, 300 mg.kg -1 ) for 21 consecutive days. Infection
more » ... e days. Infection was evaluated through skin lesions and infected footpad edema. Haematological evaluation was done on -28 th , 0 and 21 st days. Imprint footpad and lymph node weight were evaluated on 21 st day. Lb infection significantly inhibited the peripheral leukocytes blood. However, neutrophils and lymphocytes values did not have significant alterations. G3 presented eosinophilia in relation to G2. The treatment with EAE did not reduce edema of infected footpad neither weight of drainage lymph node. Infected footpad imprints revealed amastigotes forms and cellular infiltration. Animals from G3 presented skin lesions on 7 th day, shown a reduction of these lesions in day 14. Therefore, the treatment with EAE did not alter the etiological agent elimination in these conditions. However, EAE presents a healing activity in this experimental model. Keywords : Cocos nucifera hematological parameters in vivo infection immune response Leishmania braziliensis Effect of ethyl acetate extract from husk fiber water of Cocos nucifera in Leishmania braziliensis infected hamsters José C. C. Freitas et al. Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 21(6): Nov./Dec. 2011 1007 Effect of ethyl acetate extract from husk fiber water of Cocos nucifera in Leishmania braziliensis infected hamsters José C. C. Freitas et al. Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 21(6): Nov./Dec. 2011 1009
doi:10.1590/s0102-695x2011005000138 fatcat:rtvq3jsfgvde7huxzwl2eccoqm