Quantifying the optical properties of turbid media using polarization sensitive hyperspectral imaging (SkinSpect): two-layer optical phantom studies

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Anthony J. Durkin, Robert Chave, Daniel L. Farkas, Daniel L. Farkas, Dan V. Nicolau, Robert C. Leif
2015 Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII  
A polarization-sensitive hyperspectral imaging system (SkinSpect) has been built and evaluated using two-layer tissue phantoms, fabricated to mimic the optical properties of melanin in different epidermal thickness and hemoglobin in dermal layers. Multiple tissue-mimicking phantoms with varying top layer thicknesses were measured for optical system calibration and performance testing. Phantom properties were characterized and validated using SkinSpect. The resulting analysis shows that the
more » ... sed system is capable of distinguishing and differentiating the layer-dependent absorption spectra and the depths at which this absorption occurs.
doi:10.1117/12.2186031 fatcat:mrm4otpxynhcpjo2ifzh3jyp7q