Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress

Nadia O. Abutaleb, George A. Truskey
2021 STAR Protocols  
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
doi:10.1016/j.xpro.2021.100394 pmid:33796871 pmcid:PMC7995664 fatcat:ozxij657qrcxhkd5ljfsoe32qm