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Enzymatic Synthesis of multi Spin-labeled DNA
2008
Nucleic Acids Symposium Series
Electron paramagnetic resonance (EPR) spectroscopy was applied extensively in studies of nucleic acid structures and dynamics. Two modified 2'-deoxyuridine triphosphates were synthesized bearing a spin label linked to the base by a rigid linker to ensure a tight coupling of spin label dynamics. The incorporation of both spin-Iabeled nucleotides could be shown in primer extension reactions in presence of DNA polymerases from eukaryotic, prokaryotic, and archaic origin. In further experiments we
doi:10.1093/nass/nrn188
pmid:18776409
fatcat:c2nbttwdtbbqjgvigrt2b3gizu