Enzymatic Synthesis of multi Spin-labeled DNA

S. Obeid, M. Yulikov, G. Jeschke, A. Marx
2008 Nucleic Acids Symposium Series  
Electron paramagnetic resonance (EPR) spectroscopy was applied extensively in studies of nucleic acid structures and dynamics. Two modified 2'-deoxyuridine triphosphates were synthesized bearing a spin label linked to the base by a rigid linker to ensure a tight coupling of spin label dynamics. The incorporation of both spin-Iabeled nucleotides could be shown in primer extension reactions in presence of DNA polymerases from eukaryotic, prokaryotic, and archaic origin. In further experiments we
more » ... her experiments we were able to introduce multiple spin labels during primer extension reactions.
doi:10.1093/nass/nrn188 pmid:18776409 fatcat:c2nbttwdtbbqjgvigrt2b3gizu