Complementary target identification approaches for the identification of protein binding partners of phyllobilins, a family of abundant natural products degraded from chlorophyll. From senescent leaves of barley (Hordeum vulgare), the first non-fluorescent Chl catabolite (NCC), which was named phylloleucobilin (PleB) later, was identified by Kräutler and co-At the last stage of the so far investigated Chl breakdown process, pink Chl catabolites (PiCCs), which were named phylloroseobilins (PrBs)

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... recently, were also identified. The structure elucidation of a PrB extracted from the senescent leaves of the deciduous tree Cercidipyllum japonicum (Katsura tree) revealed it to be the oxidation product of Cj-PxB, featuring an additional double bond at C10/11 position [31]. Cj-PrB is the only naturally found PrB so far. However, an oxidation of the corresponding PxB by Double bonds of the basic chemical phyllobilin structure have been omitted for better illustration. Not all of the modifications are found in all different phyllobilin states (PluB, PleB, PxB, PrB). a large structural diversity of phyllobilins was observed due to modification of different side groups depending on the plant species. This structural diversity provides access to various new natural compounds and opens up the possibility of influencing polarity, solubility, and therefore cellular characteristics of phyllobilins, probably affecting cellular uptake, tissue targeting, or protein affinities. Hence, it creates a starting point for exploiting the vast source of phyllobilins as natural