Bovine Serum Albumin as A Superior "FP-Tag" for High-Throughput Glycosyltransferase Assay [post]

Xinjian Yin, Jiaxin Li, Senhua Chen, Yuping Wu, Zhigang She, Lan Liu, Yue Wang, Zhizeng Gao
2020 unpublished
<a></a>Here, we report the development of a high-throughput "FP-Tag" OGT assay with bovine serum albumin as a low-cost and superior "FP-Tag". With this assay, 2-methyleurotinone was identified as a <a></a><a>low </a>M-range OGT inhibitor. This type of assay with BSA as "FP-Tag" would find more applications with other glycosyltransferases.
doi:10.26434/chemrxiv.12840776 fatcat:g7g3g5zitndy5oaes5qiqidyfu