Genetic transformation with cell wall-associated deoxyribonucleic acid in Bacillus subtilis

R J Doyle, U N Streips, S Imada, V S Fan, W C Brown
1980 Journal of Bacteriology  
Cell walls from Bacillus subtilis 168 were prepared by conventional methods and found to contain deoxyribonucleic acid (DNA). In transfornation assays, after autolysis, it was found that two major regions of the chromosome were selectively enriched in the wall preparations. One region clustered around the replication origin and is represented by the markers purA16, ts8132, thiC5, sacA321, and hisAl. The other region included the replication terminus with representative loci metBl1, citK5,
more » ... 2, and pyrAl. All other (intemal) loci on May 8, 2020 by guest
doi:10.1128/jb.144.3.957-966.1980 fatcat:6jr3eirvbrazzjyqoizkegihie