Usefulness of Reference Materials in Calibration of Enzyme Activities

Jean-Marc Lessinger, Georges Férard, Denis Grafmeyer, Dominique Labbé, Irène Maire, Françoise Schiele, Anne Vassault
1995 Clinical Chemistry and Laboratory Medicine  
α-Amylase, alkaline phosphatase and γ-glutamyltransferase were studied in a multicentre evaluation. Analyses were performed on different patient samples. Each enzyme was assayed in two different laboratories at both 30 and 37 °C, with widely used reagent kits and with the IFCC reference method (if in existence). Results differed considerably according to the measurement procedure. Data also showed that it was not possible to employ a constant conversion factor for one enzyme and different
more » ... ques between 30 and 37 °C. Calibration with three reference materials extensively improved the intermethod consistency for most of the tested measurement procedures. It was possible to transfer accuracy from the method used for the certification of the reference material to routine procedures, by using the reference material as calibrator. Temperature did not seem to be a crucial variable for the implementation of the enzyme calibrator approach.
doi:10.1515/cclm.1995.33.11.859 fatcat:kw4wbanrnvc4na35dwcv7gcruy