Bifidobacterium faecale sp. nov., isolated from human faeces

J.-H. Choi, K. M. Lee, M.-K. Lee, C.-J. Cha, G.-B. Kim
2014 International Journal of Systematic and Evolutionary Microbiology  
A novel strain, designated strain CU3-7 T , was isolated from faeces of a two-week-old baby. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7 T was phylogenetically affiliated with members of the genus Bifidobacterium. Strain CU3-7 T showed the highest level of sequence similarity with Bifidobacterium adolescentis KCTC 3216 T (98.4 %), followed by Bifidobacterium ruminantium KCTC 3425 T (97.9 %). Analysis
more » ... hsp60 sequences showed that strain CU3-7 T was closely related to B. adolescentis KCTC 3216 T (94.0 %) and B. ruminantium KCTC 3425 T (92.5 %). The DNA-DNA hybridization values with the closely related strains were all below the cut-off value for species delineation, 17.0 % with B. ruminantium KCTC 3425 T and 14.9 % with B. adolescentis KCTC 3216 T . Fructose-6-phosphate phosphoketolase activity was detected. The predominant cellular fatty acids were C 16 : 0 (27.7 %), C 18 : 1 v9c (27.4 %) and C 18 : 1 v9c dimethylacetate (15.5 %). The DNA G+C content was 58.6 mol%. On the basis of polyphasic taxonomy, strain CU3-7 T should be classified as the type strain of a novel species within the genus Bifidobacterium, for which the name Bifidobacterium faecale sp. nov. is proposed (5KACC 17904 T 5JCM 19861 T ). Abbreviation: F6PPK, fructose-6-phosphate phosphoketolase. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, partial hsp60 and dnaJ1 gene sequences of strain CU3-7 T are KF990498, KF990499 and KF990500, respectively.
doi:10.1099/ijs.0.063479-0 pmid:24944342 fatcat:nqd2ehynrfhwbazc5i2rrjmmpa