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Establishment of reverse transcription polymerase chain reaction assay for simultaneous detection of human parainfluenza virus types 1 to 4 in children with influenza like illnesses
2018
Bratislava Medical Journal
OBJECTIVES: The aim of this study was to develop an in-house multiplex reverse transcription polymerase chain reaction (mRT-PCR), which can recognize HPIV1-4 in clinical samples. BACKGROUND: Human parainfl uenza virus (HPIV) is one of the major causes of viral respiratory infections and can affect people at any age, especially infants and young children. METHODS: Four sets of specifi c primers targeting conserved areas of hemagglutinin-neuraminidase (HN) genes of HPIV1-4, were designed and
doi:10.4149/bll_2017_145
pmid:29322811
fatcat:744zb4wbvbg77igqjob3zmzngm