Purpose: In the present study, the Poncirin was evaluated against the Paracetamol-induced liver injury using in vivo and computational approaches. Methods: The Paracetamol was administered intraperitoneally to establish the liver injury in mice and subsequently, to investigate the hepatoprotective effect of the Poncirin on the liver injury. The effect of the Poncirin was evaluated against the liver injury markers and inflammatory cytokines. Similarly, in the present study the antioxidants and

more »

... idative stress parameters were also assessed following paracetamol-induced liver injury. The histological studies following liver injury was also assessed using H and E staining, Masson's trichrome staining, and periodic acid Schiff staining. Results: The Poncirin markedly improved the antioxidant enzymes, while attenuated the oxidative stress markers and inflammatory cytokines. The Poncirin also markedly improved hematological parameters and electrolytes profile. Furthermore, the Poncirin treatment significantly improved the histological parameters using H and E staining, Masson's trichrome and PAS staining compared to the control. The Poncirin treatment also improved the liver function tests and liver synthetic activity compared to Paracetamol treated group. The immunohistochemistry analysis revealed significant decrease in the inflammatory signaling protein such as NF-κB (nuclear factor kappa light chain enhancer of activated B cells), JNK (Jun N-terminal Kinase) and COX-2 (Cyclooxygenase-2) expression level compared to the Paracetamol treated group. The in silico approach was also used to analyze the potential activity and explore the underlying molecular mechanism of Poncirin. Conclusion: The Poncirin improved the sign and symptoms associated with the liver injury using both in vivo and computational approaches.