Internet Archive Scholar

Dual polarity voltage imaging reveals subthreshold dynamics and concurrent spiking patterns of multiple neuron-types [article]

Madhuvanthi Kannan, Ganesh Vasan, Simon Haziza, Cheng Huang, Radek Chrapkiewicz, Junjie Luo, Jessica A Cardin, Mark J Schnitzer, Vincent A Pieribone
2021 bioRxiv   pre-print

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (29.8 MB)
https://web.archive.org/web/20220526155617/https://www.biorxiv.org/content/biorxiv/early/2021/10/15/2021.10.13.463730.full.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2022; you can also visit the original URL. The file type is application/pdf.

Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between distinct, targeted cell populations. However, current indicator families lack the requisite sensitivity for in vivo multipopulation imaging. We describe high-performance green and red sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants, pAce and pAceR. Our indicators enable 0.4-1 kHz voltage recordings from >50 neurons per field-of-view
more » ... awake mice and ~30-min continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered behavioral state-dependent interactions between distinct neocortical subclasses, as well as contributions to hippocampal field potentials from non-overlapping projection neuronal ensembles. By combining three mutually compatible indicators, we demonstrate concurrent triple-population voltage imaging. Our approach will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
doi:10.1101/2021.10.13.463730 fatcat:leixjcjo2ndzfemnrhdobhey6m
Citation

Madhuvanthi Kannan, Ganesh Vasan, Simon Haziza, Cheng Huang, Radek Chrapkiewicz, Junjie Luo, Jessica A Cardin, Mark J Schnitzer, Vincent A Pieribone. "Dual polarity voltage imaging reveals subthreshold dynamics and concurrent spiking patterns of multiple neuron-types." bioRxiv (2021)