Application of Low-Vacuum Scanning Electron Microscope for Vascular Bundle Observation

Y. Iokawa, S. Ogawa, T. Ohba, S. Daigobo
2001 Japanese Journal of Biological Education  
Three-dimensional observation of plant vascular bundle is important for students to understand the function of the vascular bundle as a duct of water and nutrients in connection with photosynthesis and the respiration of plants. In this study, we applied a low-vacuum scanning electron microscope (LV-SEM) college biology . Our preliminary LV-SEM observations of vascular bundles using several herbaceous dicotyledons revealed that the stem of Clematis terniflora DC. was useful as a material to
more » ... a typical vascular bundle of herbaceous dicotyledons, and it was also convenient to prepare its cross and vertical sections with a razor blade for LV-SEM observation. Vascular bundle observation using C. terniflora demonstrated that students operated LV-SEM easily, within a short period of time, and understood spatial relationship among cells constituting the vascular bundle. The present study suggests that LV-SEM
doi:10.24718/jjbe.41.2_57 fatcat:jila2hd44bar7kp7ggf56sj6jm