Molecular Characterization of Riau's Mascot Flora (Oncosperma tigillarium)

Desti, Fitmawati, Putri Ade Rahma Yulis, Mayta Novaliza Isda
2021 Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)   unpublished
This research was aims to find out about the molecular characterization of the Nibung plant (Oncosperma tigillarium). It has been conducted in the Biology Laboratory. The results of DNA isolation were tested to determine the quality of DNA that has been isolated. DNA purity was determined based on A260/A280 comparison values were tested using a nanodrop spectrophotometer (Sambrook et al. (1989) method. The quality of DNA from isolation was influenced by several factors such as the condition of
more » ... s the condition of the leaf sample used, the length of time of grinding, the phenolic content of the sample, the process of taking the supernatant solution and others. In this study, these factors were seen to significantly affect the quality of the DNA isolated. Also, DNA fragments generated from the isolation process are large, which indicates the large concentration of DNA produced. Although many DNA bands smear they still have clear DNA bands. Overall, the DNA isolation method based on the Doyle Doyle (1987) method in this study is applied to Nibung plants which is used as Riau's Province mascot flora.
doi:10.2991/assehr.k.210305.004 fatcat:fyugzztomvcibo6klfmg24uxuq