Original Article Changes in nerve fibers and microvessel density in residual spleen after subtotal splenectomy due to portal hypertension

Shengru Chen, Jianhua Zhao, Lei Wang, Yongbo Xu, Shuangshuang Zhu, Tao Wang, Kun Li, Haibo Chu
2016 Int J Clin Exp Pathol   unpublished
The precise pathogenic mechanism leading to splenic enlargement in patients with portal hy-pertension is poorly characterized. The present study was designed to determine the expression of nerve fibers and different angiogenic factors in patients with splenomegaly and residual spleens after subtotal splenectomy. Methods: Thirteen cases of splenomegaly due to portal hypertension were examined post-splenomegaly and designated as the splenomegaly group. During the ensuing 8 years we obtained other
more » ... s we obtained other specimens by puncture biopsy, which were designated as the residual spleen group. We designated 13 patients with traumatic spleen injuries as the control group. Immunohistochemistry and immunofluorescence were used to determine the expression of neuropeptide Y (NPY), neurofilament-200 (NF-200) vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGFR-2), and CD34 in residual spleens under light microscopy. Results: A significantly higher density of NPY-and NF-positive nerve fibers were observed in the splenomegaly group compared with the residual spleen and control groups (all P<0.05). Moreover, there were no significant differences between the residual spleen and control groups (P>0.05). A significantly higher microvessel density and positive expression ratio of VEGF and VEGFR-2 were observed in the splenomegaly group compared with the residual spleen and control groups (all P<0.05). There were also significant differences between the residual spleen and control groups (P<0.05). Conclusion: The residual spleen can eliminate the high-pressure state after a subtotal splenectomy, and remodeling of splenic nerves and microvessels with other splenic tissues and cells, which contributes to the recovery of splenic function.
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