Flavobacterium panaciterrae sp. nov., a β-glucosidase producing bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field

Yan Jin, Yeon-Ju Kim, Van-An Hoang, Sun Young Jung, Ngoc-Lan Nguyen, Jin Woo Min, Chao Wang, Deok-Chun Yang
2014 Journal of General and Applied Microbiology  
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69 T is JX233806. A Gram-reaction-negative, oxidase-and catalasepositive, non-gliding motile strain, designated strain DCY69 T , was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69 T were circular, 0.5 1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69 T belonged to
more » ... genus Flavobacterium with 90.5 98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C 15:0 , iso-C 17:0 3-OH, iso-C 15:0 3-OH and summed feature 3 (containing C 16:1 ω7c and/or C 16:1 ω6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69 T was 35.0 mol%. The strain DCY69 T transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69 T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69 T (= KCTC 32392 T = JCM 19161 T ), isolated from the soil of a ginseng field in the Republic of Korea.
doi:10.2323/jgam.60.59 pmid:25008165 fatcat:mimcwfvbhjd7phaqeflikipgcy