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Agonist-induced PIP2 Hydrolysis Inhibits Cortical Actin Dynamics: Regulation at a Global but not at a Micrometer Scale
2002
Molecular Biology of the Cell
Phosphatidylinositol 4, 5-bisphosphate (PIP 2 ) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP 2 sensors suggest that this lipid is enriched in membrane microdomains. Here we report that this concept needs revision. Using three distinct fluorescent GFP-tagged pleckstrin homology domains, we show that highly mobile GFP-PH patches colocalize
doi:10.1091/mbc.e02-04-0231
pmid:12221130
pmcid:PMC124157
fatcat:gkuynrm6a5e5plmmizdptyg6x4