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A biochemical test is described which reveals metabolic changes in isolated non-flagellate germinal cells of the rat testis within 4 days of treatment of the animals with sex hormones. By means of this test, a number of progestational compounds were surveyed and their minimum effective dose levels determined. Long-term treatment of male rats (100 to 400 g) with a progestin (0\m=.\5 to 2\ m=. \ 0mg/day) caused atrophy of the secondary sex glands and decreased spermatogenesis with loss of libido.doi:10.1530/jrf.0.0320453 pmid:4692341 fatcat:wbcmyedrxvbd7oklhy3hemc4ly