The effect of compression on tissue enzymes
Experimental biology and medicine
The senior author (B.H.H.) has studied various enzymes when subjected to great compression in a special hydraulic press, capable of delivering pressure of over ( I O )~ pounds per square inch. As a rule, these enzymes are inhibited or destroyed. At the same time, there is generally lee-way between the destruction of enzymes and the total destruction of bacteria included in the preparations. I t is well known from the work of investigators, such as Wolbach, Sakai and Jackson,' that it is
... that it is difficult if not impossible to obtain aseptic autolytic digests by employing the most rigid asepsis in operations of removing organs from mammals, for included bacteria are practically always present. The junior writer (W.M.) has abundantly verified this conclusion, although there exist reports of investigations, as those of Magnus-Levy,2 where the work was controlled by aerobic and anaerobic cultures and aseptic organ suspensions seem to have been obtained. We have no suggestion to make regarding this discrepancy. In order to determine whether the lee-way mentioned above is of sufficient extent to warrant an attempt to study tissue enzyme action apart from bacteria, the following experiments were conducted, rabbits being used as subjects, the livers being excised, sieved, weighed to 20 per cent. digest, 10 g. and 25 g. portions being transferred to special blocked tin tubes, resembling vaseline tubes, which were tightly stoppered by means of screw-caps, each tube then being introduced into the cylinder of the press surrounded by a jacket of water. The following table gives the number of the sample, the pressure involved, the time of exposure to this pressure and the Kjeldahl data utilized to follow the rate of digestion, if any3; the term "initial" refers to the non-protein l Wolbach, Sskai and Jackson, Journ. Med. Res., rgog, 21, 267. 'For details see Bradley and Morse, Journ. Biol. Chem., 1915, 21, 209.