Speciation of Antimony in Injectable Leishmanicidal Drugs by Lowering Citric Acid Concentration Used in Hydride Generation Atomic Absorption Spectrometry Analysis

Henrique Fabrino, Cynthia Demicheli, Frédéric Frezard, Letícia Costa
2020 Journal of the Brazilian Chemical Society  
This study proposes a procedure for speciation of antimony by hydride generation atomic absorption spectrometry in pentavalent antimony drugs. The Sb III content was determined by selective generation of SbH 3 in medium with higher Sb V concentration using 4 to 20-fold lower citric acid solutions than recommended in the available literature. The multivariate optimization of the methods was performed through factorial design followed by a central composite design (CCD). The limit of detection
more » ... D) and limit of quantification (LOQ) were calculated at 0.15 and 0.05 µg L -1 and 0.48 and 0.17 µg L -1 , for total Sb and Sb III , respectively. The relative standard deviation (RSD) values ranged from 3.1 to 19.6% and 9.1 to 20.1%, while recovery ranged from 95.6 to 102.3% and 89.1 to 108.1%, for total Sb and Sb III , respectively. This method was applied for the analysis of meglumine antimoniate samples. Total Sb and Sb III concentrations ranged from 79.2 to 101.1 mg mL -1 and 0.08 to 0.41 mg mL -1 , respectively.
doi:10.21577/0103-5053.20200148 fatcat:xdzit2sxura6tbexl2z343waba