Description of Cell Adhesion by the Langmuir Adsorption Isotherm

Karl Eckl, Hans Gruler
1988 Zeitschrift für Naturforschung C - A Journal of Biosciences  
The adhesion of granulocytes to a glass surface is both theoretically and experimentally investigated. The basic results are: (i) The adherence process of cells to a surface can be described by the Langmuir adsorption isotherm which also holds for the adsorption of molecules to a substrate. (ii) The granulocytes do not interact with each other during the adsorption/desorption process. (iii) The equilibrium constant at 37 °C for granulocytes adsorption to the glass surface is 170 cells/mm3 = 1.7
more » ... × 105 cells/ml. (iv) The equilibrium constant increases with decreasing temperature, (v) The equilibrium constant is a function of the chemokinetic/chemotactic stimulus f-Met-Leu-Phe. (vi) The desorption rate is also a function of the chemokinetic/chemotactic stimulus (kde-1 = 71.5 min for 1 nᴍ and 330 min for 1 μᴍ f-Met-Leu-Phe). (vii) The adsorption rate is a function of the chemokinetic/chemotactic stimulus and the cellular bulk concentration (kad-1- · cv-1 = 104 min for 1 nᴍ and 8 min for 1 μᴍ f-Met-Leu-Phe, and cv = 4000 cells/mm3 = 4 × 106 cells/ml), (viii) The total number of cells which can bind to the surface is independent of temperature (and of the chemokinetic/chemotactic stimulus), (ix) The high affinity receptor site is responsible for the adherence (Kf-Met- = 1.8 nᴍ f-Met-Leu-Phe). (x) The chemokinetic/chemotactic stimulated adherence is a cooperative process on a molecular level, (xi) The cellular adsorption/desorption process is a rate controlled process. The thermodynamic description of the adsorption/desorption process failed.
doi:10.1515/znc-1988-9-1022 fatcat:sm7ecdh3vnddzapx3khhgvfirq