THU0102 DENDRITIC CELLS AS A PREDICTOR OF GOOD CLINICAL RESPONSE IN RHEUMATOID ARTHRITIS PATIENTS

M. Korolev, Y. Kurochkina, N. Banshhikova, V. Omelchenko, E. Letyagina, A. Akimova, A. Lykov, O. Poveschenko
2020 Annals of the Rheumatic Diseases  
Background:Dendritic cells (DCs) are known to contribute to the pathogenesis of rheumatoid arthritis (RA) through presentation of cartilage glycoprotein, production of proinflammatory cytokines and activation of Th1/Th17 responses. DCs are a heterogeneous population and can be divided into groups: myeloid (mDCs) and plasmacytoid (pDCs). DCs can induce both immune response and tolerance.Objectives:To investigate the subpopulations of peripheral blood DCs (myeloid and plasmacytoid) in patients
more » ... oid) in patients with early RA as a predictor of responsibility to disease-modifying antirheumatic drugs (DMARDs) treatment.Methods:Fifty two patients with early RA (duration of the disease up to 12 months) were included in the study. All patients fullfield ACR/EULAR criteria (2010) and received methotrexate, leflunomide, sulfasalazine or their combination. Fifty five patients with osteoarthritis (OA) used as a control group. Analysis of the content of the B-lymphocytes, myeloid and plasmacytoid DCs was carried out by flow cytometry. B-lymphocytes, subtypes of peripheral blood DCs were characterized by the following phenotypes: myeloid DCs (CD3-CD14-CD19-HLA-DR + CD11c + CD123-), plasmacytoid DCs (CD3-CD14-CD19-HLA-DR + CD11c-CD123 +), B-lymphocytes (CD19 +). Analyses were performed before treatment and after 3 and 6 months.Results:Patients with early RA are characterized by significant evaluation of the population of myeloid DCs in comparison of patients with osteoarthritis (26.6% vs 23.5, p=0.0007). Furthermore, the difference was found in the number of cells with the phenotype B-lymphocytes: 5.4% vs 3%, p = 0.0005). No significant differences were observed in the number of plasmocytoid DCs. After 3 and 6 month of observation we detected reducing amount of myeloid DCs 26.6% vs 21.1% vs 18.4% respectively. Also we revealed reducing B-cells in treatment (5.4% vs 3 % vs 2%). Disease activity according to DAS28 droped to low (4.32 to3.06, p=0.03). We also revealed a reliable negative correlation between both the activity of the disease and the B- cells (rS=-0.4, p=0.05, n=52) and myeloid DCS (rS=-0.6, p=0.0004, n=52). A reducing of the immune cells during the DMARDS therapy suggests that they are an attractive marker for good clinical response to therapy.Conclusion:The data obtained confirm the determining role of myeloid DC and B lymphocytes in maintaining systemic inflammation in rheumatoid arthritis. In addition, these cells are a target of DMARDs therapy and a predictor of a good clinical response.Disclosure of Interests:None declared
doi:10.1136/annrheumdis-2020-eular.1392 fatcat:m6nl5xlpnbfhja2f2yoswqr7ru