Influence of CD151 Inhibition by RNAi on Lung Metastasis in Nude Mice Given Tail Veins Injection of Xuanwei Lung Adenocarcinoma XWLC-05 Cell Lines

LI Ke, WANG Chu, YANG Fang, ZENG Jiajia, ZHANG Jianqiang
2018 Zhongliu Fangzhi Yanjiu  
Objective To explore the influence of CD151 inhibition by RNA interference on invasion and metastasis of Xuanwei lung adenocarcinoma XWLC-05 cells, as well as lung metastasis in nude mice given tail veins injection of XWLC-05 cells. Methods Recombinant plasmid aimed to interfere CD151 gene was constructed and transfected into XWLC-05 cells. MTT, wound-healing assay and Transwell assay were applied to detect cell proliferation and migration abilities. Stably-expressed XWLC-05-shRNA, XWLC-05-shNC
more » ... shRNA, XWLC-05-shNC and XWLC-05-Blank cell lines were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured by naked eye and microscope after HE staining. Results Inhibition of CD151 gene expression could reduce the migration and invasion abilities of XWLC-05 cells in vitro(P < 0.05). The proportion and number of lung metastasis were 75%(6/8) and (5.2±1.2), 100%(8/8) and (9.8±1.6), 75%(7/8) and (9.1±1.2) in XWLC-05-shRNA, XWLC-05-shNC and XWLC-05-Blank groups, respectively. There was no differences in XWLC-05-shNC vs. XWLC-05-Blank group(P=0.423). There were significant differences in XWLC-05-shRNA vs. XWLC-05-shNC group(P=0.000) and XWLC-05-shRNA vs. XWLC-05-Blank group(P=0.000). Conclusion Inhibition of CD151 gene expression could reduce lung metastasis in nude mice given tail veins injection of XWLC-05 cells. CD151 may become one of the potential therapeutic targets for inhibiting the invasion and metastasis of Xuanwei lung adenocarcinoma.
doi:10.3971/j.issn.1000-8578.2018.17.1702 doaj:d50f6d2dcf1946aab00ffcdb0094a53a fatcat:dar3htbxp5d6vgn4e67s4d5q7q