The Influence of Incubation on the Wassermann Reaction

E. H. Ruediger
1918 Journal of Infectious Diseases  
Smith and MacNcal! demonstrated that widely different results may be obtained in complement-fixation for syphilis by the use of different incubation methods. First incubation conducted in the refrigerator at a low temperature gave many more positive results than did first incubation conducted at a temperature of 37 C. In a later communication" the same authors showed that cholesterinized antigen, plain alcoholic extract, and the acetone insoluble antigen bind much more complement at low
more » ... ment at low temperature than at a temperature of 37 C. In the refrigerator, plain alcoholic extract bound more complement than did cholesterinized antigen in the incubator. In this paper I shall present the results I have obtained with the Wassermann reaction under different incubation conditions. Only one antigen was employed, and the only difference there was, was in incubation time and method. . The results are presented under 5 different heads. Under the first head are given results obtained by comparing incubation in the incubator at a temperature of 37 C. with incubation in the open water-bath at a temperature of 37 C. The second deals with results obtained in the incubator at 37 C. and in the refrigerator at 10 C. Here each serum was tested 10 times, first incubation in incubator 0.25 hour, 0.5 hour, 1 hour, 2 hours and 4 hours, and these were duplicated in the refrigerator. In the third group each serum was tested 4 times; first incubation was carried on in the incubator for 1 and 4 hours, and in the refrigerator for 1 and 4 hours. In the fourth group the incubation time only was varied. First incubation was carried on in the refrigerator for 5 and 10 hours. In the fifth group first incubation at a temperature of 21 C. for 5 hours was compared with first incubation at a temperature of 10 C. for 5 hours. All human serums used in these tests were glycerolated, and in Tests 1 and 2 each tube received 0.1 c c of serum-glycerol mixture while in Tests 3, 4, and 5 each tube received 0.25 c c of serum-glycerol rnixture and the total quantity in each tube was brought up to 1.25 c c. TEST 1 Serums 1 to 20, inclusive, were tested 4 times. First incubation was in the incubator at 37 C. for 1 and 2 hours, and in the water-bath at 37 C. for 1 and 2 hours. After having added the sensitized blood corpuscles the
doi:10.1086/infdis/23.2.173 fatcat:4eos7xtlbrcj7dl6mx75nvnyjm