Von Willebrand factor binds surface-bound C1q and induces platelet rolling

Gennaro De Libero, Ed Palmer, Robert Kölm, Marten Trendelenburg
2015 unpublished
Pre-mature atherosclerosis and thrombotic complications are major causes of morbidity and mortality in patients with systemic lupus erythematosus (SLE). However, the high incidence of these complications cannot be explained by traditional risk factors alone suggesting direct effects of an activated immune system on hemostasis. The sequence homology between SLE patient-derived autoantibodies against complement C1q (Fab anti-C1q) and von Willebrand factor (vWF) suggested an interaction between
more » ... complement and hemostatic system on the level of initiating molecules. vWF was found to bind to surface-bound C1q under static conditions. The binding was specifically inhibited by Fab anti-C1q and C1q-derived peptides. Under shear stress the C1q-vWF interaction was enhanced, resembling the binding of vWF to collagen I. In addition, I could show that C1q-vWF complexes induced platelet rolling and firm adhesion. Furthermore, I observed vWF binding to C1q-positive apoptotic micro particles and increased vWF deposition in C1q-positive glomeruli of SLE patients compared to control nephropathy. I show for the first time binding of vWF to C1q and thus a direct interaction between starter molecules of hemostasis and the complement system. This direct interaction might contribute to the pathogenic mechanisms in complement-mediated, inflammatory diseases.
doi:10.5451/unibas-006416108 fatcat:jnaktdtw2fbt7kgb4hhqcy6jru