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The effect of ultrasonic irradiation on the dissociation of antigen-antibody complexes was studied. Among three frequencies tested, 28 kHz ultrasound gave a relatively high percent dissociation with the lowest decrease of immune reactivity. When dissolved antigen-antibody complexes were exposed to the ultrasonic irradiation no dissociation was observed, while antigenantibody complexes were significantly dissociated by ultrasound when the antibodies had been immobilized on CH-Sepharose 4B. Thedoi:10.1248/cpb.35.3822 fatcat:mwq4arfccbdvtgrs6fomufhmzy