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Optimization of culture media to produce Bacillus subtilis strain QST 713 in a handcrafted bioreactor
Objective. To optimize a nutrient medium based on fertilizers for the cultivation of Bacillus subtilis in an Airlift-type handcrafted bioreactor. Design/Methodology/Approach. Twenty-seven nutrient media, fixed by combining five factors with three levels, including sucrose, ammonium sulfate, triple superphosphate, UltraK® formula, and B. subtilis inoculum (Serenade® Max) were tested in a 50L handcrafted by the authors. The variables monitored in the media were absorbance, dissolved oxygen, pH,doi:10.32854/agrop.v14i9.2123 fatcat:o5zrx7ptyrfrtivfs7iisndmlq