Increasing Lymphatic Microvessel Density in Primary Pterygia

Shiqi Ling, Lingyi Liang, Haotian Lin, Weihua Li, Jiangang Xu
2012 Archives of Ophthalmology  
Objective: To examine lymphatic microvessel density (LMVD) in primary pterygia. Methods: We included tissue samples from 88 excised primary (including 34 grade 1, 28 grade 2, and 26 grade 3) pterygia and from 7 nasal epibulbar conjunctivae segments used as control samples. Sections from each pterygium were immunostained with CD31 and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) monoclonal antibodies to evaluate LMVD and blood microvessel density. We used real-time polymerase
more » ... reaction analysis to measure expression of vascular endothelial growth factor A (VEGF-A) and VEGF-C messenger RNA (mRNA) in the pterygia. Results: A small number of CD31-positive LYVE-1negative blood vessels and only a few CD31-and LYVE-1-positive lymphatic vessels were detected in the nor-
doi:10.1001/archophthalmol.2012.293 pmid:22801834 fatcat:o6b5u6gczzh3darddmg2tqiony