in the 【 immcrtmxis of 鰰 9 沈 艪 砿 er融 ) en 囲 simUli a 毘 SEnsed by tbttr transmembrmne chemor 叩 tOrs 〔 MCPs )and an M ¢ P . lil {e し ransmenlbrane prote血 ( the redaxsenmAET ) . Reg皿 dlt / ss 匸 heirsignalingstateE , MCPs andAerexistashDmodimers and 士 brm a temmry complenc with tho histidine kma se CheA and the adaptr } r CheW 、 ' 11iey fbrm a hage clし 旧 肥 rat a U pDle 血 atrimer MCP 〔 andAer )dim a8 abUildingunit . IleeceptOr clus し e 血 g has been implicatud 血 . Signal nmplification a . nd gain con

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... 劬 1 、 However , mal 曲 meChanis 田 s 【 「 frtlrepderelu . abe血 9 dp 。lfiTlocali7atian aveonlypporly し mdcTstood , In this stUay . we examined 出 e formation 。 f P 由 r dus 婢 u 曲 9 [ 祖 伽 恥 n . de臼 cieni . Tar mut 乏 mts 出 at have 曲 9且 e amino aCid substitUtions in 出 e HAMP dDma 血 which cロnnects the transmembrane helix ti っ 士 he adapta 恒 o ゴ8 脚 ng doma血. Wb fJund 匸 hat lmlo . r loca ] ization of Tar mutantG were res 〔uedb ン the co ・ expre 蕊 bnofAe 弓 which 帆 1 曲 副 蜘 by i厩 11 . .加 晦 臼 . link囃 ays to probe the 門 t血 er dirnorE " suggested that 亡 he mo 就 Eevere 願 hr mu 七 ation imrmaires 出e fOrmatiOn andbr structUre of 出 e trimer 血 er . 「 Mhus, the a ] ity的 敵 m a cluster can be伽 d by a st 甑 圜 d ges 血 are 蜘 n ( θ. 厚 the HMW domnirO 。ther tban 働 e II 価 mer oD 劇 . 11 which is at the tip of 出e 蜘 p1蹴 domain . Thoso resultg a ] so 5uppo 助 し he no 口on し haC e 脳 ctiveduste 血 ユ giSreq し 血 edforthepolm ' 1mhtionofM ( ) P ( andpresumab 且 } ・ Aer) .