The cellular distribution of estrogen receptor-α and -β mRNAs in the reproductive organs of the rat : An in situ hybridization study

Mowa, Chishimba Nathan
immunoreactivity for insulin, while a cells increased in number. The second part of the study aimed to elucidate the difference, with respect to the existence of a CaBP, which is known to buffer Ca 2 +rises following stimulation. Calbindin-D 28 k is a predominant calcium-binding protein contained in the pancreatic islets. It can buffer Ca 2 + rises following stimulation and thereby protect cells against calcium toxicity. The immunoreactivity for calbindin-D 28 k was localized only in a cells in
more » ... only in a cells in normal mice, but not present in ~ cells. This finding held true in islet cells of the KATP channel-deficient mice. An immunohistochemical survey using six rodents including the mouse showed that calbindin-D 28 k was preferentially localized in a cells in the rat and guinea pig, while in the hamster both a and ~ cells were rich in calbindin-D 28 k. None of the a and ~ cells in the squirrel or gerbil pancreas were immunoreactive for calbindin-D 28 k. This finding may explain how a cells but not ~ cells in the knockout mice could escape from the calcium toxicity, and shows that the cellular localization of calbindin-D 28 k in the islets differs even among rodents. The cellular distribution of estrogen receptor a (ER a) and ~ (ER~) mRNAs in the reproductive organs of the rat: an in situ hybridization study This study thoroughly mapped the cellular distribution of estrogen receptor (ER) a and ER ~ mRNAs in the reproductive organs of the female and male rats by in situ hybridization technique in order to elucidate the roles of estrogen in the reproductive events. In the adult female rat, expression of ER a and ER ~ mRNAs was predominant in the reproductive tract and ovary, respectively. ER a mRNA had the most pronounced expression in epithelial cells and subepithelial stromal cells from the oviduct to the vagina, while in the ovary it was moderately detected in only the theca folliculi and interstitial glands. The oviduct showed a region-dependent expres-sion pattern ofER a mRNA: the isthmus had the most intense signals while the infundibulum revealed a low intensity of expression. Signals for ER ~ mRNA in the ovary were most intense in the granulosa cells of healthy follicles whereas degenerating follicles lacked any significant expression. Less intense signals for ER ~ mRNA were localized in the theca folliculi and corpus luteum. Detectable levels of ER ~ mRNA were observed in the subepithelial stromal cells from the oviduct to the vagina. In the developing female reproductive organs, diffuse signals of ER a and ~ mRNAs were coexpressed in the fetal ovary; they
doi:10.11501/3182498 fatcat:bxdveadb25hqlhiu23bmodepnu