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Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime
2012
Journal of Biomedical Optics
Photoacoustic (PA) microscopy (PAM) can image optical absorption contrast with ultrasonic spatial resolution in the optical diffusive regime. Conventionally, accurate quantification in PAM requires knowledge of the optical fluence attenuation, acoustic pressure attenuation, and detection bandwidth. We circumvent this requirement by quantifying the optical absorption coefficients from the acoustic spectra of PA signals acquired at multiple optical wavelengths. With the acoustic spectral method,
doi:10.1117/1.jbo.17.6.066011
pmid:22734767
pmcid:PMC3381032
fatcat:mltswb7nybaz7pebkoyeqqdjy4