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Biochemical analysis of nucleosome targeting by Tn5 transposase
2019
Open Biology
Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. However, in chromatin, the DNA targeting by Tn5 transposase has remained unclear. In the present study, we reconstituted well-positioned 601 dinucleosomes, in which two nucleosomes are connected with a linker DNA, and studied the DNA integration sites in the dinucleosomes by Tn5 transposase in vitro. We found that Tn5
doi:10.1098/rsob.190116
pmid:31409230
pmcid:PMC6731594
fatcat:xetifey3vbcxvfvut3xbc6kl44