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We produced a hybridoma secreting an immunoglobulin Gl monoclonal antibody against the circumsporozoite protein of the human malaria parasite Plasmodium malariae (Uganda 1/CDC). The monoclonal antibody produces a circumsporozoite precipitation reaction when incubated with viable sporozoites of P. malariae and reacts at high titers with heat-fixed sporozoites in an indirect immunofluorescent antibody test. Using the purified monoclonal antibody and Western blot analysis, we identified twodoi:10.1128/iai.45.3.592-595.1984 fatcat:nipo2q5ptrbtjejac5jsl6hf4y