Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury

Xiao-Qing Tang, Yun Wang, Ji-Sheng Han, You Wan
2001 NeuroReport  
The protective effects of adenovirus-mediated glia cell linederived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose± and time±response relationship of glutamate neurotoxicity was determined on spinal motoneuron cultures. Then, the effect of the gdnf recombinant adenovirus (AdCMVgdnf) was tested in this cellular model. AdCMVgdnf at 20 MOI (multiplicity of infection) was found to signi®cantly reduce the cell loss of motoneurons, as compared to
more » ... AdCMVgdnf at 20 MOI, the recombinant adenovirus containing the marker gene lacZ. Furthermore, the adenovirus was proved to mediate erogenous gene expression using X-Gal staining and a semi-quantitative RT-PCR method. These results suggested a therapeutic potential of adenovirus vector-mediated gdnf gene therapy in human motoneuron diseases.
doi:10.1097/00001756-200110080-00018 pmid:11568639 fatcat:idnabuhofvattiskcwymoanhwa