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Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle
1996
Journal of Virology
Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fragments (SFv) which bind to the HIV-1 reverse transcriptase (RT) enzyme. The expression of anti-RT SFv in Tlymphocytic cells specifically neutralizes the RT activity in the
doi:10.1128/jvi.70.6.3392-3400.1996
fatcat:2z3lv3qmvrcsldruhluqtxrb4a