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Engineered biosynthetic pathways have the potential to produce high-value molecules from inexpensive feedstocks, but a key limitation is engineering enzymes with high activity and specificity for new reactions. Here, we developed a method for combining structure-based computational protein design with library-based enzyme screening, in which interresidue correlations favored by the design are encoded into a defined-sequence library. We validated this approach by engineering a glucose 6-oxidasedoi:10.1016/j.chembiol.2010.10.012 pmid:21168766 fatcat:2mbqudqofzcvfhmjim52p7ngle