Mettl14 is required for mouse post-implantation development by facilitating epiblast maturation [article]

Qing-Yuan Sun, Tie Gang Meng, Xukun Lu, Lei Guo, Guan Mei Hou, Qian Nan Li, Xue Shan Ma, Lin Huang, Li Hua Fan, Zheng Hui Zhao, Xiang Hong Ou, Ying Chun Ouyang (+3 others)
2018 bioRxiv   pre-print
N6-methyladenosine (m6A) is the most prevalent and reversible internal modification of mammalian messenger and noncoding RNAs mediated by specific m6A writer, reader, and eraser proteins. As an m6A writer, the METTL3-METTL14-WTAP complex dynamically regulates m6A modification and plays important roles in diverse biological processes. However, our knowledge about the complete functions of this RNA methyltransferase complex, the contributions of each component to the methylation and their impacts
more » ... on different biological pathways, are still very limited. Here, by employing both in vivo and in vitro models, we report that METTL14 was indispensable for post-implantation embryonic development by facilitating the conversion from naive to primed state of the epiblast. Depletion of Mettl14 lead to conspicuous embryonic growth retardation from E6.5 mainly as a result of resistance to differentiation, which further lead to embryonic lethality early in gestation. Our data highlight the critical function of METTL14, as an m6A modification regulator, in orchestrating early mouse embryogenesis.
doi:10.1101/294926 fatcat:2e643irhi5h7xkfmibsm4lw4pe