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Epidermal stem cells isolation struggle remains, mainly due to the yet essential requirement of well-de fi ned approaches and markers. The herein proposed methodology integrates an assemblage of strategies to accomplish the enrichment of the interfollicular epidermal stem cells multipotent fraction and their subsequent separation from the remaining primary human keratinocytes culture. Those include rapid adherence of freshly isolated human keratinocytes to collagen type IV through the βdoi:10.1007/7651_2018_146 pmid:29980942 fatcat:t5pvgezvqzgolpqffus6qc4zue