Bcl-xLInhibits CytochromecRelease but Not Mitochondrial Depolarization during the Activation of Multiple Death Pathways by Tumor Necrosis Factor-α

Bryan W. Johnson, Enrique Cepero, Lawrence H. Boise
2000 Journal of Biological Chemistry  
Cells can respond differently to anti-CD95 antibody treatment. Type I cells show strong activation of caspase-8 and directly activate caspase-3. Type II cells weakly activate caspase-8 and must amplify their death signal through the mitochondria. These cells can be rescued by Bcl-x L . Here we show that tumor necrosis fac- tor-␣ induces both Type I and II pathways, which can be inhibited by benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-fmk) and Bcl-x L in a cooperative fashion. Death
more » ... tive fashion. Death induced in the presence of Z-VAD-fmk was associated with a partial inhibition of caspase-8, whereas no effects on cytochrome c release, DEVDase activity, and intranucleosomal DNA cleavage were observed. Thus, Z-VAD-fmk is likely weakening the deathinducing signaling complex-mediated activation of caspase-8 and diverting cells to a Type II pathway. Bcl-x L cooperates with Z-VAD-fmk by blocking the Type II pathway at the level of cytochrome c release. Surprisingly, although Bcl-x L was able to block cytochrome c release, it was unable to block mitochondrial depolarization, suggesting that these are separate events. This suggests that mitochondria occupy two places in apoptotic signaling, as initiators of apoptosis through the release of cytochrome c as well as a target for effector caspases.
doi:10.1074/jbc.m001363200 pmid:10913120 fatcat:ou6e7tszsffojamldcjakkka4i