Gene regulation by sense-antisense overlap of polyadenylation signals

R. Gu, Z. Zhang, J. N. DeCerbo, G. G. Carmichael
2009 RNA: A publication of the RNA Society  
We show here that expression of genes from convergent transcription units can be regulated by the formation of doublestranded RNA (dsRNA) in the region of overlapping polyadenylation signals. The model system employed is the mouse polyomavirus. The early and late genes of polyomavirus are transcribed from opposite strands of the circular viral genome. At early times after infection, the early genes are expressed predominantly. Late gene expression increases dramatically upon the onset of DNA
more » ... lication, when a major defect in polyadenylation of the late primary transcripts generates multigenomic RNAs that are precursors to the mature late mRNAs. Embedded in these late pre-mRNAs are sequences complementary to the early RNAs that act to down-regulate early gene expression via A-to-I editing of dsRNAs. In this system, the defective polyadenylation, and consequently the production of multigenomic late RNAs, depends on the context, and perhaps also, on the A-to-I editing of the poly(A) signal that overlaps the 39-end of early transcripts. . Article published online ahead of print. Article and publication date are at
doi:10.1261/rna.1608909 pmid:19390116 pmcid:PMC2685520 fatcat:rux5ydcaafaaxjq2g6xfe5rcxm