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Convenient Isolation and Kinetic Mechanism of Glutathionylspermidine Synthetase fromCrithidia fasciculata
Journal of Biological Chemistry
Trypanothione, the essential metabolite in the oxidant defense system of trypanosomatids, is synthesized by two distinct proteins, glutathionylspermidine synthetase and trypanothione synthetase. Glutathionylspermidine synthetase was purified to homogeneity from the trypanosomatid Crithidia fasciculata by aqueous two-phase systems and chromatography. The enzyme showed a specific activity of 38 mol of glutathionylspermidine formed per min per mg of protein. Its molecular mass was 78 kDa indoi:10.1074/jbc.272.18.11908 pmid:9115252 fatcat:vdjpsygjz5auxfhqnlfmfcdf7e