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tRNA Recognition of tRNA-guanine Transglycosylase from a Hyperthermophilic Archaeon,Pyrococcus horikoshii
2000
Journal of Biological Chemistry
In the biosynthesis of archaeosine, archaeal tRNAguanine transglycosylase (TGT) catalyzes the replacement of guanine at position 15 in the D loop of most tRNAs by a free precursor base. We examined the tRNA recognition of TGT from a hyperthermophilic archaeon, Pyrococcus horikoshii. Mutational studies using variant tRNA Val transcripts revealed that both guanine and its location (position 15) were strictly recognized by TGT without any other sequence-specific requirements. It appeared that
doi:10.1074/jbc.m005043200
pmid:11060284
fatcat:wtevsyczarbu7flgelireej4vy