Resolution Limits in the Study of Cardiolipin Crystals by TEM, SAED and AFM

Giovanni Valdrè, Umberto Muscatello, Ugo Valdrè
1995 Microscopy Microanalysis Microstructures  
2014 Aggregates of cardiolipin molecules have been studied by transmission electron microscopy (TEM), selected area electron diffraction (SAED) and atomic force microscopy (AFM). It is found that cardiolipin molecules are very susceptible to electron damage; the lethal dose being of 80 el·nm-2. The finest organized structures revealed by TEM after image recording with the minimum dose technique are rather coarse, about 1 nm, whereas SAED reveals the presence of periodicities down to 0.2 nm, in
more » ... down to 0.2 nm, in accordance to the images obtained by AFM, whose application has enabled us to reveal substructures of a size of about 0.2 nm. Microsc. Microanal. Microstruct. 6 (1995) Classification Physics Abstracts
doi:10.1051/mmm:1995155 fatcat:7g46wdpjozcipplgxqzy33yrj4