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In vitro propagation of Morus alba L. in semisolid culture medium

Enrique Salas Barbosa, Daniel Agramonte, Raúl Barbón, Felipe Jiménez, Raúl Collado, Martha Pérez, Odalys Gutiérrez
2005 Biotecnología Vegetal  

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Apical buds as explants were used with the objective to propagate in vitro mulberry plants in semisolid MS culture medium suplemented with 6-BAP and KIN in their establishment and, with different combinations of 6-BAP with ANA in the multiplication phase. In vitro plants were evaluated during the acclimatization phase. It is necessary to supplement the basal MS culture media with 0.5 mg.l-1 of 6-BAP to induce the sprouting and, 0.5 mg.l-1 of 6-BAP and 0.5 mg.l-1 of ANA to multiply the mulberry
more » ... y nodal segments. In the acclimatization phase a 95% of survival, 30.2 cm of height, 9.8 leaves and 2.02 g.plant-1 of dry mass was observed. In vitro propagation of mulberry was achieved as an alternative for plants production. Key words: acclimatization, apical buds, establishment, explant, shooting
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Enrique Salas Barbosa, Daniel Agramonte, Raúl Barbón, Felipe Jiménez, Raúl Collado, Martha Pérez, Odalys Gutiérrez. "In vitro propagation of Morus alba L. in semisolid culture medium." Biotecnología Vegetal (2005)