This research investigates the effect of mixing wavelength light photoperiods (12 h blue, 8 h blue: 4 h green, 4 h blue: 8 h green, and 12 h green) and N/P ratios (1.3 to 8.3) on the growth microalgae–bacteria systems, organic matter, and nutrient removals. The highest microalgae–bacteria growth performance (μ = 0.2 d−1, 481.1 ± 15.3 mg DW L−1) was observed when a 8 h blue: 4 h green mixed wavelength and a low N/P ratio were used. For both N/P ratios, biomass productivity was favored when using

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... the blue light dominated at longer time periods. Mechanisms for nitrogen removal by assimilation depend on the N/P ratio, achieving assimilation between 49 and 65% at a low N/P ratio. High nitrogen removal (>50%) showed a strong relation with alkalinity culture conditions (pH > 8.5). The mixing of wavelength photoperiods seems to be a promising strategy to achieve high biomass productivity and nutrient removal. However, for optimal conditions, N/P ratios in the wastewater should be considered.