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Periploca aphylla is used in the treatment of many diseases ethno pharmacologically in Pakistan. In the present manuscript, we demonstrate the antimicrobial effects of P. aphylla. Six different bacteria (Escherichia coli, Bacillus subtilis, Micrococcus luteus, Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa) are used in the screening of extract through agar well diffusion technique as well as four different strains of fungi (Fusarium solani, Aspergillus niger,doi:10.5897/jmpr11.1112 fatcat:ftfw66u4enghveipvsdkwjn4nq